Reena Rahayu Md Zin who had worked hard on giving ideas. National Center for Biotechnology Information, U. S. National Library of Medicine 8600 Rockville Pike, Bethesda MD, 20894 USA1Pattern Recognition Research Group, Center for Artificial Intelligence Technology, Faculty of Information Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia2Department of Pathology, UKM Medical Center, Universiti Kebangsaan Malaysia, Cheras, 56000 Kuala Lumpur, MalaysiaSegmentation and counting of blood cells are considered as an important step that helps java programming extract features java programming diagnose some true ailments like malaria or leukemia. The manual counting of white blood cells WBCs and red blood cells RBCs in microscopic images is an extremely tedious, time consuming, and misguided method. Automatic evaluation will allow hematologist specialists java programming carry out faster and more accurately.